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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which results in chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was discovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical purpose in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two additional splice variants had been noted but thorough nucleotide sequence information and facts is not really nevertheless obtainable.
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Blocking peptides are peptides that bind especially towards the target antibody and block antibody binding. These peptide commonly is made up of the epitope identified because of the antibody.
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This guide outlines further more attainable optimization of cellular and immunoassay parameters to be sure the absolute best final results are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining through the blocked antibody as opposed to the antibody by yourself, you can see which staining is distinct.
Goal Info Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile click here cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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